Mouse RENIN Protein, His Tag

分子別名（Synonym）

REN,FLJ10761,Renin,angiotensinogenase

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Mouse RENIN, His Tag (REN-M5222) is expressed from human 293 cells (HEK293). It contains AA Leu 22 - Arg 402 (Accession # NP_112469.1). The propeptide sequence is AA Leu 22 - Thr 71.

Predicted N-terminus: Ser 72

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 37.0 kDa. The protein migrates as 47-57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

腎素也稱為REN和血管緊張素原酶，是一種參與人體腎素-血管緊張素系統(tǒng)（RAS）的循環(huán)酶，在動(dòng)脈血壓升高和腎臟鈉潴留增加中起著至關(guān)重要的作用。腎素通過(guò)切割肝臟產(chǎn)生的血管緊張素原來(lái)激活腎素-血管緊張素系統(tǒng)，產(chǎn)生血管緊張素I，血管緊張素II進(jìn)一步被血管緊張素轉(zhuǎn)換酶ACE轉(zhuǎn)化為血管緊張素Ⅱ，ACE主要位于肺毛細(xì)血管內(nèi)。腎素由腎細(xì)胞分泌，腎細(xì)胞通過(guò)致密斑的信號(hào)傳導(dǎo)被激活，致密斑對(duì)通過(guò)遠(yuǎn)端小管的液體流速做出反應(yīng)，通過(guò)腎灌注壓的降低（通過(guò)血管壁中的牽張受體），以及交感神經(jīng)刺激，主要是通過(guò)β-1受體激活。腎素可以與ATP6AP2結(jié)合，導(dǎo)致血管緊張素原向血管緊張素I的轉(zhuǎn)化比可溶性腎素增加四倍。此外，腎素結(jié)合導(dǎo)致ATP6AP2的絲氨酸和酪氨酸殘基磷酸化。腎素mRNA的水平似乎受到HADHB、HuR和CP1與3'UTR調(diào)節(jié)區(qū)結(jié)合的調(diào)節(jié)。過(guò)度活躍的腎素-血管緊張素系統(tǒng)會(huì)導(dǎo)致血管收縮和鈉和水的滯留。這些影響會(huì)導(dǎo)致高血壓。因此，腎素抑制劑可用于治療高血壓。