Biotinylated Human VSIG4 Protein, His,Avitag? (MALS verified)
分子別名（Synonym）
VSIG4,CRIg,Z39IG
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Biotinylated Human VSIG4 Protein, His,Avitag (VS4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 283 (Accession # Q9Y279).
Predicted N-terminus: Arg 20
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 32.8 kDa. The protein migrates as 43-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
標(biāo)記（Labeling）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
含V-set和免疫球蛋白結(jié)構(gòu)域的蛋白4（VSIG4）也稱為蛋白Z39Ig，是一種I型跨膜糖蛋白。VSIG4是B7家族相關(guān)蛋白和Ig超家族成員。VSIG4包含兩個(gè)Ig樣（免疫球蛋白樣）結(jié)構(gòu)域。VSIG4在幾種胎兒組織中大量表達(dá)。在成人組織中，VSIG4在肺和胎盤中的表達(dá)最高。VSIG4作為T細(xì)胞活化的負(fù)調(diào)節(jié)因子發(fā)揮作用，可能參與維持外周T細(xì)胞耐受，也被確定為已建立炎癥的強(qiáng)效抑制劑。VSIG4是一種吞噬受體，是T細(xì)胞增殖和IL2產(chǎn)生的強(qiáng)負(fù)調(diào)節(jié)因子。
關(guān)鍵字： VSIG4;VSIG4蛋白;VSIG4重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺(tái)型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個(gè)城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計(jì)服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。