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PGRMC1抗體,Mouse Monoclonal PGRMC1 Antibody
  • PGRMC1抗體,Mouse Monoclonal PGRMC1 Antibody
  • PGRMC1抗體,Mouse Monoclonal PGRMC1 Antibody
  • PGRMC1抗體,Mouse Monoclonal PGRMC1 Antibody

PGRMC1抗體

價格 詢價
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 湖北
更新日期 2025-05-10
QQ交談 微信洽談

產(chǎn)品詳情

中文名稱:PGRMC1抗體英文名稱:Mouse Monoclonal PGRMC1 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 866 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類別: 抗體
重組: 應用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 靶點: PGRMC1
2025-05-10 PGRMC1抗體 Mouse Monoclonal PGRMC1 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 866 year. Avoid repeated freeze / thaw cycles. 抗體

驗證與應用

應用及物種
WB咨詢技術(shù) Human,Mouse,Rat
IF咨詢技術(shù) Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesMPR; HPR6.6
Entrez GeneID10857
clone6F9A1
WB Predicted band size21.7kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human PGRMC1 (AA:1-195) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of MCF-7 cells using PGRMC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of SK-OV-3 cells using PGRMC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of A549 cells using PGRMC1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PGRMC1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using PGRMC1 mouse mAb with DAB staining.    


           

參考文獻

以下是關(guān)于PGRMC1抗體的3篇參考文獻,包含文獻名稱、作者及摘要概括:

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1. **文獻名稱**: *"Progesterone Receptor Membrane Component 1 (PGRMC1) Associates with EGFR and Regulates Erlotinib Sensitivity"*

**作者**: Ahmed IS et al. (2012)

**摘要**: 該研究利用特異性PGRMC1抗體進行Western blot和免疫共沉淀實驗,發(fā)現(xiàn)PGRMC1通過與EGFR相互作用調(diào)控非小細胞肺癌細胞對厄洛替尼的敏感性,高表達PGRMC1與患者不良預后相關(guān)。

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2. **文獻名稱**: *"Optimization of PGRMC1 Antibody Selection for Immunohistochemistry in Reproductive Tissues"*

**作者**: Friberg PA et al. (2016)

**摘要**: 研究對比了多種市售PGRMC1抗體在人類卵巢和子宮內(nèi)膜組織中的特異性,通過免疫組化和siRNA敲減驗證抗體可靠性,為生殖醫(yī)學研究提供抗體選擇依據(jù)。

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3. **文獻名稱**: *"PGRMC1 Modulates Chemoresistance and Cellular Stress Response in Glioblastoma"*

**作者**: Pellegrino R et al. (2018)

**摘要**: 通過免疫熒光和流式細胞術(shù),研究揭示PGRMC1在膠質(zhì)母細胞瘤中通過調(diào)控自噬和氧化應激通路增強化療耐藥性,特異性抗體驗證了其在腫瘤組織中的高表達。

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4. **文獻名稱**: *"PGRMC1 in Ovarian Follicle Development: Antibody Validation and Functional Analysis"*

**作者**: Hughes AL et al. (2020)

**摘要**: 研究驗證了PGRMC1抗體在小鼠卵泡顆粒細胞中的特異性,結(jié)合基因敲除模型,證實PGRMC1在類固醇合成和卵母細胞成熟中的關(guān)鍵作用。

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以上文獻均明確使用PGRMC1抗體進行機制或臨床相關(guān)性研究,覆蓋癌癥、生殖生物學等領(lǐng)域。

       

背景信息

Progesterone Receptor Membrane Component 1 (PGRMC1) is a non-classical membrane-associated progesterone receptor belonging to the MAPR family, characterized by a cytochrome b5-like heme-binding domain. Initially identified for its role in steroidogenesis, PGRMC1 is implicated in diverse cellular processes, including cell cycle regulation, apoptosis, cholesterol biosynthesis, and drug metabolism. It localizes to intracellular membranes (endoplasmic reticulum, Golgi) and the plasma membrane, interacting with signaling proteins like EGFR and CYP enzymes. Dysregulation of PGRMC1 is linked to cancer progression, neuropathologies (e.g., Alzheimer’s), and reproductive disorders, driving interest in its mechanistic studies.

PGRMC1 antibodies are essential tools for detecting protein expression, post-translational modifications (e.g., phosphorylation), and subcellular localization. Commonly used in Western blotting, immunofluorescence, and immunohistochemistry, these antibodies target epitopes within its N-terminal domain or C-terminal region. However, specificity challenges arise due to PGRMC1's homology with PGRMC2 and splice variants. Validation via knockout controls or siRNA-mediated silencing is critical. Commercial antibodies vary in performance across applications, necessitating context-specific optimization. Recent studies also highlight PGRMC1's role in chemoresistance, making its antibodies valuable for therapeutic research in oncology and metabolic diseases.

       
關(guān)鍵字: PGRMC1抗體;PGRMC1;PGRMC1 Antibody;

公司簡介

湖北瑞和寧生物科技有限公司研究創(chuàng)新能力, 擁有完整、科學、質(zhì)量管理體系。并不斷致力于技術(shù)創(chuàng)新,產(chǎn)品創(chuàng)新和管理創(chuàng)新,贏得了國內(nèi)外廣大用戶的信任。
成立日期 2024-06-24 (2年) 注冊資本 50萬(元)
員工人數(shù) 1-10人 年營業(yè)額 ¥ 100萬以內(nèi)
主營行業(yè) 中間體,化學試劑 經(jīng)營模式 貿(mào)易,工廠,試劑,定制,服務
  • 湖北瑞和寧生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊資本:50萬(元)
  • 企業(yè)類型:有限責任公司(自然人投資或控股)
  • 主營產(chǎn)品:中間體。試劑
  • 公司地址:江漢區(qū)
詢盤

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