The HSPA1L antibody targets the heat shock protein family A (HSP70) member 1-like (HSPA1L), a constitutively expressed chaperone protein involved in cellular stress responses, protein folding, and immune modulation. HSPA1L, located on chromosome 6p21.3. shares high homology with inducible HSP70 isoforms (HSPA1A/HSPA1B) but is regulated differently, maintaining basal expression under non-stress conditions. It plays roles in maintaining proteostasis, aiding antigen presentation, and protecting cells from apoptosis during thermal or oxidative stress.
Antibodies against HSPA1L are essential tools for studying its expression, localization, and function in diseases. Researchers use them in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate HSPA1L's involvement in cancer, neurodegenerative disorders (e.g., Alzheimer’s), and inflammatory conditions. Elevated or dysregulated HSPA1L has been linked to tumor progression, chemoresistance, and neuroprotection, making it a potential therapeutic or diagnostic target.
HSPA1L polymorphisms, particularly in the promoter region, are associated with disease susceptibility, including male infertility and infection outcomes. Antibodies help validate these associations by correlating genetic variants with protein expression levels. Commercial HSPA1L antibodies are typically validated for specificity using knockout controls or siRNA, though cross-reactivity with homologous HSP70 proteins requires careful interpretation. Overall, HSPA1L antibodies advance understanding of stress response mechanisms and their pathological implications.