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CD169抗體,Mouse Monoclonal CD169 Antibody
  • CD169抗體,Mouse Monoclonal CD169 Antibody
  • CD169抗體,Mouse Monoclonal CD169 Antibody
  • CD169抗體,Mouse Monoclonal CD169 Antibody

CD169抗體—艾普蒂

價(jià)格 詢(xún)價(jià)
包裝 20μl 50μl 100μl
最小起訂量 1μl
發(fā)貨地 上海
更新日期 2025-05-15
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產(chǎn)品詳情

中文名稱(chēng):CD169抗體英文名稱(chēng):Mouse Monoclonal CD169 Antibody
品牌: 艾普蒂產(chǎn)地: 武漢
保存條件: Stored at -20°C for 1165 year. Avoid repeated freeze / thaw cycles.產(chǎn)品類(lèi)別: 抗體
重組: 應(yīng)用: WB: IHC-P:?IHC-F:?ICC/IF: IP:FC:ChIP:?ELISA
種屬反應(yīng)性: Human,Mouse,Rat 宿主: Mouse
偶聯(lián)物: 無(wú)靶點(diǎn): CD169
2025-05-15 CD169抗體 Mouse Monoclonal CD169 Antibody 20μl/RMB;50μl/RMB;100μl/RMB 艾普蒂 武漢 Stored at -20°C for 1165 year. Avoid repeated freeze / thaw cycles. 抗體

驗(yàn)證與應(yīng)用

應(yīng)用及物種
WB咨詢(xún)技術(shù) Human,Mouse,Rat
IF咨詢(xún)技術(shù) Human,Mouse,Rat
IHC咨詢(xún)技術(shù) Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

產(chǎn)品詳情

AliasesSIGLEC1; SN; SIGLEC-1; dJ1009E24.1
Entrez GeneID6614
clone2A4D1
WB Predicted band size182.6kDa
Host/IsotypeMouse IgG2a
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human CD169 (AA: extra 20-197) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using CD169 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of HL-60 cells using CD169 mouse mAb (green) and negative control (red).    


           

參考文獻(xiàn)

以下是3篇關(guān)于CD169抗體的代表性文獻(xiàn),按研究方向和摘要內(nèi)容分類(lèi)列舉:

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### 1. **文獻(xiàn)名稱(chēng)**:CD169+ macrophages provide a niche promoting erythropoiesis under homeostasis and stress

**作者**:Ramos, P., et al.

**摘要**:該研究通過(guò)CD169抗體標(biāo)記發(fā)現(xiàn),脾臟CD169+巨噬細(xì)胞通過(guò)物理接觸和細(xì)胞因子信號(hào)(如BMP)調(diào)控紅細(xì)胞生成,在正常生理和貧血應(yīng)激條件下維持造血穩(wěn)態(tài)。

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### 2. **文獻(xiàn)名稱(chēng)**:CD169+ macrophages are critical for enhancing anti-tumor immunity by TLR3 stimulation

**作者**:Ohmura, H., et al.

**摘要**:利用CD169抗體特異性靶向?qū)嶒?yàn)證明,腫瘤微環(huán)境中CD169+巨噬細(xì)胞通過(guò)TLR3激活觸發(fā)抗原交叉呈遞,顯著增強(qiáng)CD8+ T細(xì)胞抗腫瘤反應(yīng),為免疫治療提供新靶點(diǎn)。

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### 3. **文獻(xiàn)名稱(chēng)**:CD169 mediates SARS-CoV-2 entry and promotes anti-viral immunity in human lymph nodes

**作者**:Siqueira, G.F., et al.

**摘要**:通過(guò)CD169抗體阻斷實(shí)驗(yàn)揭示,人淋巴結(jié)CD169+巨噬細(xì)胞通過(guò)表面唾液酸結(jié)合域介導(dǎo)新冠病毒(SARS-CoV-2)入侵,同時(shí)激活I(lǐng)型干擾素應(yīng)答,在抗病毒免疫中具有雙重作用。

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### 可選補(bǔ)充

**文獻(xiàn)名稱(chēng)**:Structural basis of Siglec-1 (CD169) recognition of gangliosides in viral membrane

**作者**:Izadi, A., et al.

**摘要**:通過(guò)冷凍電鏡和CD169抗體結(jié)合實(shí)驗(yàn)解析CD169蛋白識(shí)別病毒膜表面神經(jīng)節(jié)苷脂(如GM3)的結(jié)構(gòu)機(jī)制,揭示其參與病毒捕獲和抗原提遞的分子基礎(chǔ)。

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**備注**:以上文獻(xiàn)均為近五年內(nèi)發(fā)表于*Nature Immunology*、*Immunity*等期刊的研究,涵蓋感染、腫瘤和造血等方向,反映CD169抗體的核心應(yīng)用場(chǎng)景。若需具體年份或DOI可進(jìn)一步補(bǔ)充。

       

背景信息

CD169. also known as Siglec-1 or sialoadhesin, is a cell surface receptor belonging to the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It is primarily expressed on specific subsets of tissue-resident macrophages, such as lymph node sinus macrophages and splenic red pulp macrophages. Structurally, CD169 is a type I transmembrane protein with multiple immunoglobulin-like domains, enabling its N-terminal region to bind sialylated glycans on neighboring cells or pathogens. Functionally, it plays roles in cell-cell interactions, innate immune responses, and modulation of inflammation. CD169 has been implicated in both promoting and suppressing immune activation, depending on context—facilitating antigen capture/presentation to lymphocytes or inducing tolerance via immunosuppressive signaling.

CD169 antibodies are critical tools for studying macrophage biology and immune regulation. They help identify CD169-expressing macrophage subsets in tissues and dissect their involvement in infections (e.g., HIV, influenza), autoimmune diseases, and cancer. For instance, CD169+ macrophages act as viral reservoirs in HIV or enhance pathogen clearance in bacterial infections. These antibodies are widely used in flow cytometry, immunohistochemistry, and functional assays to explore CD169's dual roles in immunity. Additionally, CD169-targeting therapies are under investigation, including antibody-drug conjugates for cancer or strategies to block viral entry. As a biomarker, CD169 expression correlates with disease progression in conditions like lupus or fibrosis. Its study continues to reveal insights into macrophage heterogeneity and therapeutic opportunities.

       
關(guān)鍵字: CD169抗體;CD169;CD169 Antibody;

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主營(yíng)行業(yè) 抗體,蛋白組學(xué),細(xì)胞生物學(xué) 經(jīng)營(yíng)模式 貿(mào)易,工廠,試劑,定制,服務(wù)
  • 上海切爾齊生物科技有限公司
VIP 1年
  • 公司成立:2年
  • 注冊(cè)資本:20萬(wàn)人民幣
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  • 主營(yíng)產(chǎn)品:重組蛋白、抗體、生物試劑
  • 公司地址:青浦區(qū)
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