**Background of PPM1K Antibody**
PPM1K (Protein Phosphatase Mg2?/Mn2?-Dependent 1K), also known as PP2Cκ or POPCK, is a member of the PPM family of serine/threonine phosphatases that require Mg2? or Mn2? for catalytic activity. It plays a critical role in regulating mitochondrial metabolism, particularly in the catabolism of branched-chain amino acids (BCAAs) by dephosphorylating and activating the branched-chain α-keto acid dehydrogenase (BCKDH) complex. Dysregulation of PPM1K is linked to metabolic disorders, including diabetes, cardiovascular diseases, and neurological conditions.
Antibodies targeting PPM1K are essential tools for studying its expression, localization, and function in cellular pathways. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate PPM1K’s role in stress responses, energy homeostasis, and disease mechanisms. For instance, studies have utilized PPM1K antibodies to explore its interaction with AMP-activated protein kinase (AMPK) and mTOR signaling pathways, which are central to metabolic regulation.
Commercial PPM1K antibodies are typically developed in rabbits or mice, using immunogenic peptides or recombinant protein fragments. Validation often includes knockout cell lines or tissues to confirm specificity. Research-grade antibodies help uncover PPM1K’s therapeutic potential, particularly in modulating BCAA metabolism and mitigating metabolic syndrome-related pathologies.