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     Western blot analysis of Phospho-Histone H3 (Thr3) in HeLa cell lysates treated with FBS + Calyculin A.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human tonsil, using Phospho-Histone H3 (T3) Antibody.    

  
  

The Phospho-Histone H3 (Thr3) antibody is a specialized tool used to detect histone H3 phosphorylated at threonine 3 (H3T3ph), a post-translational modification associated with chromatin dynamics during cell division. Histone H3. a core component of nucleosomes, undergoes phosphorylation at specific residues to regulate chromatin condensation and chromosome segregation in mitosis. While phosphorylation at serine 10 (S10) or serine 28 (S28) is more commonly linked to mitotic progression, Thr3 phosphorylation has been implicated in early mitotic events, such as the activation of the Aurora B kinase complex at centromeres. This modification may also play a role in epigenetic regulation by influencing interactions with chromatin-binding proteins.

The antibody is widely utilized in cell biology and cancer research to study mitotic activity, particularly in tissues or cultured cells. It serves as a marker for mitotic cells in techniques like immunofluorescence, flow cytometry, or immunohistochemistry, aiding in the assessment of proliferation rates in tumors or drug response studies. Specificity validation (e.g., peptide blocking assays) is critical, as cross-reactivity with other phospho-epitopes can occur. Research using this antibody has contributed to understanding cell cycle dysregulation in diseases, including cancer, where aberrant mitotic signaling is a hallmark. Its applications extend to developmental biology and neurobiology, exploring cell proliferation in tissue-specific contexts.