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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Phospho-Tau (T231) expression in (1) SH-SY5Y cell lysate; (2) SH-SY5Y cell lysate, treated with sorbitol.    

  
  

Phospho-Tau(T231) antibody is a specialized tool used to detect tau protein phosphorylated at threonine 231 (T231), a key post-translational modification implicated in neurodegenerative diseases. Tau, a microtubule-associated protein, stabilizes neuronal microtubules in normal physiology. However, hyperphosphorylation of tau disrupts this function, leading to its aggregation into neurofibrillary tangles (NFTs), a hallmark of Alzheimer’s disease (AD) and other tauopathies. Phosphorylation at T231 is an early event in tau pathology, associated with conformational changes that promote detachment from microtubules and subsequent misfolding. This site is also linked to tau’s interaction with Pin1. a prolyl isomerase that may regulate tau’s functional state or degradation.

The Phospho-Tau(T231) antibody is widely used in research to study disease mechanisms, monitor tau pathology progression, and evaluate therapeutic interventions in models of AD, chronic traumatic encephalopathy (CTE), and related disorders. It is employed in techniques like Western blotting, immunohistochemistry, and ELISA to quantify phosphorylated tau levels in brain tissues, cerebrospinal fluid (CSF), or blood-based biomarkers. Elevated T231-phosphorylated tau in CSF correlates with early AD stages, making it a potential diagnostic marker. Commercial versions of this antibody are typically validated for specificity using knockout controls or phosphatase-treated samples. Its role in distinguishing pathological tau from normal tau underscores its importance in both basic research and clinical applications targeting tau-driven neurodegeneration.