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     Western blot analysis of BubR1 expression in HepG2 cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The BubR1 antibody is a crucial tool in studying the BubR1 protein (also known as BUB1B), a key component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. BubR1 plays a vital role in monitoring kinetochore-microtubule attachments and delaying anaphase until all chromosomes are properly aligned. Dysregulation of BubR1 is linked to mitotic errors, aneuploidy, and diseases such as cancer or developmental disorders like mosaic variegated aneuploidy syndrome.

BubR1 antibodies are widely used in research to detect and quantify BubR1 protein levels, assess its localization via immunofluorescence, and investigate its interactions with other SAC components (e.g., Mad2. Cdc20) or regulatory kinases. These antibodies are essential for exploring BubR1's roles in cell cycle regulation, aging (reduced BubR1 correlates with age-related pathologies), and cancer biology (overexpression or mutations in tumors).

Commercial BubR1 antibodies are typically developed in hosts like mice or rabbits, targeting specific epitopes (e.g., N-terminal or kinase domains). Validation includes Western blotting, immunoprecipitation, and knockout cell line controls. Challenges include cross-reactivity with related kinases (e.g., BUB1) or detecting splice variants. Recent studies also highlight BubR1's potential as a therapeutic target or biomarker in cancers with chromosomal instability. Its study continues to shed light on mitosis, genome integrity, and disease mechanisms.