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     Western blot analysis of PRPF31 expression in Jurkat cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

**Background of PRPF31 Antibody**

PRPF31 (pre-mRNA processing factor 31) is a core component of the spliceosome, a large ribonucleoprotein complex essential for pre-mRNA splicing. It plays a critical role in recognizing the 3' splice site during the assembly of the U4/U6.U5 tri-snRNP complex, ensuring accurate removal of introns and proper mRNA maturation. Mutations in the *PRPF31* gene are linked to autosomal dominant retinitis pigmentosa (adRP), a hereditary retinal degenerative disease causing progressive vision loss. The pathogenicity is attributed to haploinsufficiency, where reduced protein levels impair spliceosomal function, particularly in stress-prone retinal cells.

PRPF31 antibodies are essential tools for studying the protein's expression, localization, and interaction networks. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate PRPF31's role in splicing mechanisms and disease pathology. These antibodies help identify tissue-specific expression patterns, quantify protein levels in disease models, and validate CRISPR-edited or siRNA-treated cells. Commercially available PRPF31 antibodies are typically raised against epitopes within conserved regions, with validation across species (e.g., human, mouse). Their application extends to clinical research, aiding in understanding retinal degeneration mechanisms and exploring therapeutic strategies for *PRPF31*-linked adRP.