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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Meckelin expression in (1) MCF7 cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.    

  
  

Meckelin antibodies target the Meckelin protein (encoded by the *TMEM67* gene), a transmembrane glycoprotein critical for primary cilia function and cellular signaling pathways. Meckelin is localized to the transition zone of primary cilia, structures essential for sensing extracellular signals and regulating developmental processes. Mutations in *TMEM67* are linked to ciliopathies, notably Joubert syndrome (JBTS), Meckel-Gruber syndrome (MKS), and nephronophthisis, characterized by neurological, renal, and hepatic abnormalities. These disorders stem from disrupted ciliary structure or signaling, leading to impaired organogenesis and tissue homeostasis.

Meckelin antibodies are valuable tools in research to study cilia-related pathologies. They enable the visualization and quantification of Meckelin expression in tissues or cell cultures via techniques like immunohistochemistry, Western blotting, and immunofluorescence. Such studies help elucidate Meckelin's role in ciliogenesis, cell polarity, and Hedgehog signaling. Additionally, these antibodies aid in diagnosing ciliopathies by identifying protein expression anomalies in patient samples. Recent advances in CRISPR and organoid models have further highlighted Meckelin's interactions with other ciliary proteins, driving therapeutic exploration for ciliopathies. Overall, Meckelin antibodies bridge molecular insights with clinical applications, enhancing understanding of ciliary biology and disease mechanisms.