The histone H3 (formylK122) antibody is a specialized tool used to detect the post-translational formylation of lysine 122 on histone H3. a modification implicated in epigenetic regulation and DNA damage response. Histones, including H3. undergo various chemical modifications (e.g., acetylation, methylation, phosphorylation) that modulate chromatin structure and gene expression. Formylation, a less-characterized modification, involves the addition of a formyl group to lysine residues. K122 formylation is proposed to influence nucleosome stability, DNA-histone interactions, or serve as a signaling mark during cellular stress. This antibody specifically recognizes the formyl group at K122. enabling researchers to study its spatial-temporal distribution, mechanistic role in chromatin dynamics, and potential links to diseases like cancer or neurodegenerative disorders. Its development often involves immunizing hosts with synthetic formylK122-containing peptides, followed by affinity purification and validation via techniques such as ELISA, Western blotting, or immunofluorescence. Researchers use this antibody to explore formylation's crosstalk with other histone modifications, its regulation by enzymes (e.g., formyltransferases or deformylases), and its functional impact on transcription, DNA repair, or replication. Ongoing studies aim to clarify its biological significance, leveraging this antibody as a critical reagent in epigenetics and chromatin biology.