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     Western blot analysis of Phospho-GRLF1 (Tyr1087) in 293T lysates using Phospho-GRLF1 (Tyr1087) antibody.    

  
  

The Phospho-GRLF1 (Tyr1087) antibody is a specialized tool used to detect the phosphorylation status of Guanine Nucleotide Releasing Factor 1 (GRLF1) at tyrosine residue 1087. GRLF1. also known as RasGRF1 or CDC25. is a member of the RasGEF family that activates Ras signaling by promoting the exchange of GDP for GTP. This protein plays a role in regulating cell growth, differentiation, and synaptic plasticity, particularly in neuronal and endocrine tissues. Phosphorylation at Tyr1087 is a critical post-translational modification linked to GRLF1 activation, often triggered by upstream signals such as receptor tyrosine kinases (e.g., EGFR, insulin receptor). This modification enhances its GEF activity, facilitating Ras-MAPK pathway activation and downstream cellular responses.

The Phospho-GRLF1 (Tyr1087) antibody is widely used in biomedical research to study signaling dynamics in conditions like cancer, neurological disorders, and metabolic diseases. It enables specific detection of the activated form of GRLF1 in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Validation typically includes testing in knockout models or phosphorylation-inhibited samples to confirm specificity. Researchers rely on this antibody to explore mechanisms of Ras pathway dysregulation, drug-target interactions, or biomarker discovery, making it essential for understanding GRLF1-related signaling networks in health and disease.