天堂网亚洲,天天操天天搞,91视频高清,菠萝蜜视频在线观看入口,美女视频性感美女视频,95丝袜美女视频国产,超高清美女视频图片

ChemicalBook >> journal list >> Journal of Chromatography A >>article
Journal of Chromatography A

Journal of Chromatography A

IF: 3.79
Download PDF

A rapid method for detecting bronopol in fresh fish, shrimp, crab, and shellfish samples using liquid chromatography-tandem mass spectrometry

Published:8 November 2023 DOI: 10.1016/j.chroma.2023.464429 PMID: 37826921
Mengqiang Wang , Li Jia , Xun Jiao , Tangwei Zhang , Ming Xiao , Miao Wang , Shanshan Wang , Lufei Zheng , Jing Wang , Yongxin She , A.M. Abd?EI-Aty

Abstract

Fish farming plays a vital role in providing food, nutrition, and employment globally. However, this industry faces security challenges, necessitating the use of fungicides and preservatives, such as bronopol, to increase product yields. Bronopol (2?bromo-2-nitropropan-1,3-diol; CAS:52–51–7) is widely used in various fields, including food production, cosmetics, and, more recently, aquaculture. Currently, there is a limited number of techniques available for detecting bronopol in aquaculture products. This is primarily due to bronopol's instability, susceptibility to degradation, and tendency to form precipitates that pose challenges in extraction from aquaculture products. For this issue, this study presents a comprehensive method for detecting bronopol content in aquaculture tissues using liquid chromatography-tandem mass spectrometry (LC?MS/MS). The methodology was optimized, involving extraction with Cu-Zn precipitant, cleanup using a small HLB column, separation on a T3 column, and gradient elution with water and acetonitrile mobile phases. The quantitative approach was employed without the use of an internal standard, following the external standard method. The spiked recoveries at 3 fortification levels (0.1, 0.2, and 1?mg/kg) ranged from 87.1?% to 108.1?% with relative standard deviations RSD ≤ 9.0?%. By applying this method to fresh fish, shrimp, crab, and shellfish samples from a local supermarket, no residues of bronopol were detected, ensuring the reliability of the results. The simplicity, rapidity, and high sensitivity of the method make it a suitable alternative to conventional techniques for bronopol detection. Moreover, the successful validation of the method's recovery and precision supports its potential application in monitoring and preventing the misuse of bronopol in aquaculture, thereby safeguarding aquaculture product quality and protecting public health.

Substances (3)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Acetonitrile 75-05-8 C2H3N 1052 suppliers $61180.00
Bronopol 52-51-7 C3H6BrNO4 843 suppliers $11.65-$2495.47
SOD1 / Cu-Zn SOD Antibody (aa2-154, Cy3) LS-C701675 - Inquiry