72657-23-9
72657-23-9 結(jié)構(gòu)式
基本信息
(R)-(-)-3-羥基異丁酸甲酯
(2R)-3-羥基-2-甲基丙酸甲酯
BETA-羥基異丁酸甲酯
(R)-(-)-3-羥基-2-甲基丙酸甲酯
D-(R)-(-)-3-HYDROXYISOBUTYRIC ACID METHYL ESTER
(-)-METHYL D-BETA-HYDROXYISOBUTYRATE
METHYL D-(R)-3-HYDROXYISOBUTYRATE
METHYL (R)-(-)-3-HYDROXY-2-METHYLPROPIONATE
METHYL (R)-3-HYDROXY-2-METHYLPROPIONATE
METHYL (R)-(-)-3-HYDROXYISOBUTYRATE
(R)-(-)-3-HYDROXY-2-METHYLPROPIONIC ACID METHYL ESTER
(R)-(-)-3-HYDROXYISOBUTYRIC ACID METHYL ESTER
(R)-(-)-METHYL 3-HYDROXY-2-METHYLPROPIONATE
[R]-[-]-METHYL 3-HYDROXY-2-METHYL-PROPIONATE
(R)-(-)-METHYL B-HYDROXYISOBUTYRATE
(R)-(-)-3-hydroxy-2-methylpropionic*acid methyl E
Methyl (2R)-3-hydroxy-2-methyl-propanoate
Methyl-D-(R)-beta-hydroxyisobutyrate
METHYL (R)-(-)-3-HYDROXY-2-METHYL-PROPIO NATE, 99% (99% EE/GLC)
(R)-(-)-3-HYDROXY-2-METHYLPROPIONIC*ACID METHYL EST
Propanoic acid, 3-hydroxy-2-methyl-, methyl ester, (2R)-
(-)-methyl d-β-hydroxyisobutyrate
(2R)-3-Hydroxy-2-methylpropionic Acid Methyl Ester
物理化學(xué)性質(zhì)
制備方法
15484-46-5
72657-23-9
一般步驟：以2-(羥甲基)丙烯酸甲酯為原料合成(-)-甲基-D-BETA-羥基異丁酸酯的一般程序如下：Ene-還原酶活性測定需在環(huán)境溫度下于厭氧條件（Coy Laboratory，Grass Lake，MI）中進(jìn)行。反應(yīng)原液的制備包括將各底物（具體見下文）溶解于50mM Tris-HCl緩沖液（pH7.5）中，并補(bǔ)充200μM NADP+、100mM葡萄糖及來自Thermoplasma acidophilum的葡萄糖脫氫酶（GDH）（IVTT反應(yīng)使用2個單位，純化酶反應(yīng)使用5個單位）。選擇單個底物濃度以確保達(dá)到vmax條件或最大溶解度。在這些底物濃度下，調(diào)整反應(yīng)時間以達(dá)到10-50%的底物轉(zhuǎn)化率。對于IVTT實(shí)驗(yàn)中的OYE活性測定，將20μL反應(yīng)原液與2-10μL IVTT反應(yīng)混合物混合，并用50mM Tris-HCl（pH7.5）將總測定體積調(diào)整至30μL。對于純化的OYE1變體的測定，將酶（最終濃度：250nM）加入至500μL反應(yīng)儲備溶液中。通過從測定溶液中取出30μL等分試樣，并與30μL含有1mM環(huán)己酮（作為內(nèi)標(biāo)）的乙酸乙酯充分混合以淬滅反應(yīng)進(jìn)程。收集有機(jī)相樣品并通過氣相色譜（GC）進(jìn)行分析（具體方案見下文）。通過積分產(chǎn)物和底物的峰面積來計(jì)算對映體/非對映異構(gòu)體過量。各底物的相對轉(zhuǎn)化率通過將測得的OYE1變體的轉(zhuǎn)化率除以野生型OYE1的相應(yīng)速率來計(jì)算。
參考文獻(xiàn)：
[1] Advanced Synthesis and Catalysis, 2015, vol. 357, # 8, p. 1775 - 1786
[2] Tetrahedron Asymmetry, 2009, vol. 20, # 2, p. 210 - 213
[3] Angewandte Chemie - International Edition, 2009, vol. 48, # 12, p. 2162 - 2165
[4] Advanced Synthesis and Catalysis, 2008, vol. 350, # 16, p. 2533 - 2543
[5] Advanced Synthesis and Catalysis, 2010, vol. 352, # 14-15, p. 2663 - 2666
| 報價日期 | 產(chǎn)品編號 | 產(chǎn)品名稱 | CAS號 | 包裝 | 價格 |
| 2025/05/22 | 43618 | (R)-(-)-3-羥基異丁酸甲酯 Methyl (R)-(-)-beta-hydroxyisobutyrate, 98%, Thermo Scientific Chemicals | 72657-23-9 | 5g | 2252元 |
| 2025/05/22 | H0703 | (R)-(-)-3-羥基異丁酸甲酯 Methyl (R)-(-)-3-Hydroxyisobutyrate | 72657-23-9 | 5G | 770元 |
| 2025/05/22 | H0703 | (R)-(-)-3-羥基異丁酸甲酯 Methyl (R)-(-)-3-Hydroxyisobutyrate | 72657-23-9 | 25G | 2825元 |