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7385-67-3

中文名稱 尼羅紅
英文名稱 NILE RED
CAS 7385-67-3
EINECS 編號(hào) 230-966-0
分子式 C20H18N2O2
MDL 編號(hào) MFCD00011639
分子量 318.37
MOL 文件 7385-67-3.mol
更新日期 2025/08/13 18:05:45
7385-67-3 結(jié)構(gòu)式 7385-67-3 結(jié)構(gòu)式

基本信息

中文別名
尼羅絲
尼羅紅
英文別名
9-(DIETHYLAMINO)-5H-BENZO[A]PHENOXAZIN-5-ONE
NILE BLUE A OXAZONE
NILE RED
9-(diethylamino)-5h-benzo[a]phenoxazin-5-on
nile
9-(diethylamino)benzo[a]phenoxazin-5(5H)-one
FLUORESCENCE QY-STANDARD E CERTIFIED
Fluorescent Spectral Standard E, certified
9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on
Nile Red, pure, 99%
9-Diethylamino-benzo[a]phenoxazin-5-one
7-(Diethylamino)-1,2-[1,3]butadieno-3H-phenoxazine-3-one
9-Diethylamino-5H-benzo[a]phenoxazine-5-one
Oxazine O-17M
Phenoxazone
所屬類別
化學(xué)試劑:多環(huán)化合物

物理化學(xué)性質(zhì)

熔點(diǎn)203-205 °C(lit.)
熔點(diǎn)203-205 °C(lit.)
沸點(diǎn)457.53°C (rough estimate)
密度1.1871 (rough estimate)
折射率1.5700 (estimate)
儲(chǔ)存條件2-8°C
儲(chǔ)存條件room temp
溶解度在甲醇中可溶1mg/mL
酸度系數(shù)(pKa)4.08±0.20(Predicted)
形態(tài)粉末
顏色深紅色至栗色
外觀Green to Dark Green Solid
最大波長(zhǎng)(λmax)553 nm
BRN279110
穩(wěn)定性穩(wěn)定的。與強(qiáng)氧化劑不相容。
InChIInChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChIKeyVOFUROIFQGPCGE-UHFFFAOYSA-N
SMILESC12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1
CAS 數(shù)據(jù)庫(kù)7385-67-3(CAS DataBase Reference)

安全數(shù)據(jù)

危險(xiǎn)性符號(hào)(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險(xiǎn)性描述H302-H315-H319-H335
安全說(shuō)明24/25
安全說(shuō)明S24/25
WGK Germany3
WGK Germany3
海關(guān)編碼29349990

應(yīng)用領(lǐng)域

用途1
生化研究

常見(jiàn)問(wèn)題列表

熒光染料
尼羅紅Nile Red是一種理想的脂質(zhì)熒光染料,對(duì)甘油三酯,膽固醇親和力高,結(jié)合后脂滴可發(fā)出強(qiáng)熒光信號(hào),可用于多種類型的樣本。尼羅紅具有環(huán)境敏感性熒光。尼羅紅在富脂環(huán)境中熒光強(qiáng)烈,而在水介質(zhì)中熒光微弱。尼羅紅是熒光顯微鏡和流式細(xì)胞熒光法檢測(cè)細(xì)胞內(nèi)脂滴的極好的重要染色劑。尼羅紅將細(xì)胞內(nèi)脂滴染成紅色,熒光波長(zhǎng) 559/635 nm。
尼羅紅染色
尼羅紅是一種親脂性熒光染色劑,它是一類環(huán)境敏感性熒光染料,能夠在富含脂質(zhì)的環(huán)境中產(chǎn)生強(qiáng)烈的熒光,而在水性介質(zhì)中的熒光強(qiáng)度極小,這種特性使得它成為了一款極重要檢測(cè)細(xì)胞內(nèi)脂滴的熒光指示劑,可以通過(guò)熒光顯微鏡和流式細(xì)胞檢測(cè)結(jié)果。

2)染色步驟
① 尼羅紅染色液配制:a.母液準(zhǔn)備:取適量的尼羅紅(Mw:318.37g/mol)用無(wú)水DMSO充分溶解制備1mM儲(chǔ)存液,按照單次用量分裝凍存,避免反復(fù)凍融,避光保存。b.工作液準(zhǔn)備:用HHBS或生理緩沖液(pH 7.0)將母液按1:1000稀釋為1×尼羅紅工作液,漩渦混勻。
② 用檢測(cè)化合物處理細(xì)胞一段時(shí)間;
③ 離心并調(diào)整細(xì)胞濃度為1-5×10^5cell/管;
④ 用500μL尼羅紅工作液重懸細(xì)胞;
⑤ 室溫或37oC避光孵育5-10min;
⑥ 吸掉染色工作液,用HHBS或適當(dāng)緩沖液清洗細(xì)胞;
⑦ 用500μL預(yù)熱的HHBS或培養(yǎng)基重懸細(xì)胞,使細(xì)胞密度為1-5×105cell/管;
⑧ 熒光顯微鏡或流式細(xì)胞儀檢測(cè)熒光信號(hào),Ex/Em=552/636nm。

【注意】:① 對(duì)于貼壁細(xì)胞,可用HHBS或適當(dāng)緩沖液清洗細(xì)胞,然后直接加入尼羅紅工作液孵育;② 細(xì)胞也可預(yù)固定,然后用尼羅紅工作液染色。
生物活性
Nile Red (Nile Blue A oxazone, Phenoxazone 9)是一種十分有效的熒光染料,可在疏水環(huán)境下檢測(cè)細(xì)胞內(nèi)脂質(zhì)滴 intracellular lipid droplets。Nile Red 可用于細(xì)胞內(nèi)脂質(zhì),蛋白質(zhì)的疏水域和溶酶體磷脂內(nèi)含體的染色。
靶點(diǎn)
TargetValue
lipid droplet
()
體外研究

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.

體內(nèi)研究

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.

知名試劑公司產(chǎn)品信息

尼羅紅價(jià)格(試劑級(jí))
報(bào)價(jià)日期產(chǎn)品編號(hào)產(chǎn)品名稱CAS號(hào)包裝價(jià)格
2025/05/2241571尼羅紅
Nile Red, 99%, pure
7385-67-3100mg1159元
2025/05/2241571尼羅紅
Nile Red, 99%, pure
7385-67-31g5634元
2025/05/22HY-D0718尼羅紅
Nile Red
7385-67-35 mg250元
"7385-67-3" 相關(guān)產(chǎn)品信息
536-90-3 99-07-0 93-05-0 7385-67-3 15799-79-8 538-51-2 91-68-9 94-70-2