7385-67-3

基本信息
尼羅紅
NILE BLUE A OXAZONE
NILE RED
9-(diethylamino)-5h-benzo[a]phenoxazin-5-on
nile
9-(diethylamino)benzo[a]phenoxazin-5(5H)-one
FLUORESCENCE QY-STANDARD E CERTIFIED
Fluorescent Spectral Standard E, certified
9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on
Nile Red, pure, 99%
9-Diethylamino-benzo[a]phenoxazin-5-one
7-(Diethylamino)-1,2-[1,3]butadieno-3H-phenoxazine-3-one
9-Diethylamino-5H-benzo[a]phenoxazine-5-one
Oxazine O-17M
Phenoxazone
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
常見(jiàn)問(wèn)題列表
2)染色步驟
① 尼羅紅染色液配制:a.母液準(zhǔn)備:取適量的尼羅紅(Mw:318.37g/mol)用無(wú)水DMSO充分溶解制備1mM儲(chǔ)存液,按照單次用量分裝凍存,避免反復(fù)凍融,避光保存。b.工作液準(zhǔn)備:用HHBS或生理緩沖液(pH 7.0)將母液按1:1000稀釋為1×尼羅紅工作液,漩渦混勻。
② 用檢測(cè)化合物處理細(xì)胞一段時(shí)間;
③ 離心并調(diào)整細(xì)胞濃度為1-5×10^5cell/管;
④ 用500μL尼羅紅工作液重懸細(xì)胞;
⑤ 室溫或37oC避光孵育5-10min;
⑥ 吸掉染色工作液,用HHBS或適當(dāng)緩沖液清洗細(xì)胞;
⑦ 用500μL預(yù)熱的HHBS或培養(yǎng)基重懸細(xì)胞,使細(xì)胞密度為1-5×105cell/管;
⑧ 熒光顯微鏡或流式細(xì)胞儀檢測(cè)熒光信號(hào),Ex/Em=552/636nm。
【注意】:① 對(duì)于貼壁細(xì)胞,可用HHBS或適當(dāng)緩沖液清洗細(xì)胞,然后直接加入尼羅紅工作液孵育;② 細(xì)胞也可預(yù)固定,然后用尼羅紅工作液染色。
Target | Value |
lipid droplet
() |
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.
知名試劑公司產(chǎn)品信息
Nile Red, pure, 99%(7385-67-3)
Nile Red(7385-67-3)