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Analytical Chemistry

Analytical chemistry is the subject for the method and basic principle of studying and identifying of the composition, status, structure of matter as well as determination of related content. It is an important branch of chemistry subject. Analytical chemistry plays an important role in not only its own development but also in various fields related to the chemistry. We can say that all the practice of any human activity involving chemical phenomena is inseparable from analytical chemistry. Now, people have developed various kinds of different analytical methods, which can be classified based on an analysis task, analysis object, the basis of the analysis, requirement of the analysis and sample dosage.

According to the analysis tasks, it can be divided into qualitative analysis, quantitative analysis and structural analysis. Task of qualitative analysis is to identify the elements, radicals, functional groups or compounds that constituting the substances; the task of the quantitative analysis is to determine the content of the related components in the sample; the task of structural analysis is to study the molecular structure or crystal structure of the material.

(1) According to the analysis objects, it can be divided into organic and inorganic analysis; the object for the inorganic analysis is inorganic substance; the object of organic analysis is organic substance. In the inorganic analysis, it is generally required to determine what elements, ions, radicals or compounds that constitutes the sample and measure the percentage of each component; and sometimes it is also necessary for determination of the crystal structure; in the organic analysis, it not only requires the identification of the constituent elements, but also, more importantly, should do the structure analysis and functional group analysis.
(2) According to whether the analysis is based on the physical properties or chemical properties of the substance, it can be divided into instrumental analysis and chemical analysis. Depending on the specific requirements, it can be divided into routine analysis, rapid analysis and arbitration Analysis. Routine analysis refers to the general daily laboratory production analysis, also known as conventional analysis; rapid analysis is a kind of routine analysis and is mainly applied to the control of the production process, demanding the report of the results in the shortest possible time with the error generally being allowed to be greater; arbitration analysis is needed when there is controversy in the analysis results from different institute, demanding related department to conduct accurate analysis using specific method in order to determine the accuracy of the results of the original analysis.
(3) According to the amount of the sample, it can be generally divided into constant (> 0.1g), semi-micro (0.01 ~ 0.1g) and trace (1 ~ 10mg) analysis.
(4) In the inorganic qualitative chemical analysis, people generally apply semi-micro method while people generally apply constant analysis in the quantitative chemical analysis. According to the relative amounts of the analyzed components contained in the sample, it is also roughly divided into constant component analysis (> 1%), minor component analysis (0.01 to 1%) and trace components analysis (<0.01%). For the analysis of some trace amount of components contained in some kinds of complicated mixture and some substances, it is necessary to perform separation and enrichment. This produces a series of separation techniques, such as extraction, distillation, ion exchange, chromatography, sedimentation and flotation separation, these chemical separation techniques are an integral part of the analysis.

Environmental Analytical Chemistry
Environmental Analytical Chemistry is briefly referred to environmental analysis. It is a kind of subject to study the types, components of pollutants in the environment as well as how to perform qualitative and quantitative analysis on the chemical contaminants in the environment. It is a branch of environmental chemistry.

Environmental analytical chemistry emerged, developed and improved during the process of solving environmental problems. In 1950s, the public nuisance disease occurred in Japan had alerted the whole world. In order to find the cause of public nuisance disease, after experiencing as long as 11 years, later, the chemists of environmental analysis had applied light spectrum and identified that the river in Itai-itai disease area contained harmful elements such as lead, cadmium, arsenic and so on. Further by means of tracking element analysis of the soil and food in the disease area, people had found high lead and cadmium content. Later, people had further conducted spectral quantitative analysis on the body and bone of the patients in the disease area and found that the bone ashes contained alarmingly high content of zinc, lead and cadmium. To determine the causative agent, people further incorporated zinc, lead and cadmium into the food for feeding animals and conduct trace elemental analysis for animals and confirmed the serious harm of cadmium on the bone, revealing the cause of the Itai-itai disease. The development of modern science, especially the development of modern chemistry, physics, mathematics, electronics, biology, as well as the emergence of accurate, reliable, sensitive, selective, rapid, simple environmental pollution analysis technology and automation equipment, has been resulting in the maturation of environmental analytical chemistry. Environmental analytical chemistry now has penetrated into all areas of the entire environmental science subject. It is the most effective means of access to environmental information quality.
The objects of the environmental analytical chemistry research are quite complicated, including air, water, soil, sediment, minerals, waste, animals, plants, food, and human tissue. The content of chemical elements or compound to be determined in the environmental analytic chemistry is very low, with the absolute content being within the level of 10-6 to 10-12 grams.


The analysis technology in the environmental analytical chemistry is developing towards the direction of continuous automation, computerization and joint combination of various methods and instruments. Currently applied automatic analysis methods include colorimetric analysis, ion selective electrode, x-ray fluorescence spectroscopy, atomic absorption spectroscopy, polarography, gas chromatography, liquid chromatography and flow injection analysis. Laser, as the light source of analytical chemistry technique, has also been applied. Since the laser analysis has properties of high resolution, high sensitivity, long-range and short-term, the laser technology will play a pivotal role in the development of environmental analytical chemistry.

With the deepened development of environmental science, environmental analytical chemistry is often demanded for trace levels and ultra-trace-level detection and analysis, therefore, high sensitivity. Thus study of analysis methods of high sensitivity, good selectivity, rapid trace and ultra trace will become the major development direction for environmental analysis in the near future.

Qualitative Analysis of Chemistry
Qualitative analytic chemistry is the subject to identify the chemical elements and atoms groups contained in the sample. It is a branch subject of the analysis chemistry. Its purpose is to ascertain the chemical composition of the research object (specimen).
The major research content of the qualitative analytic chemistry includes:

1 the tested samples were analyzed separately. Namely take part of the sample and use exclusive reaction to detect a desire detection component.
2 systematic analysis of the samples. This means successively apply a few selective reactions for gradual separation of the ions followed by separation of each group until separating to only one substance and finally apply confirming reaction to ascertain the existence of this substance. The most famous cation system analysis method is H2S system. In recent years, due to the use of advanced equipment, qualitative analysis has also rapidly developed together with multivariate analysis and has also become an important direction for analytical chemistry.

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Structure Chemical Name CAS MF
Avibactam Impurity 44 Avibactam Impurity 44 2064219-22-1 C23H46N4O6S
Bortezomib Impurity W Bortezomib Impurity W
Butyphthalide impurity 43 Butyphthalide impurity 43 952412-99-6 C11H14O3
Cabozantinib impurity 23 Cabozantinib impurity 23 406-00-8 C6H6FNO
Celecoxib Impurity 17 Celecoxib Impurity 17 2247197-65-3 C17H14F3N3O4S
Dapoxetine impurity 21 Dapoxetine impurity 21
Febuxostat Impurity 74 Febuxostat Impurity 74
Febuxostat Impurity 79 Febuxostat Impurity 79
Febuxostat Impurity 94 Febuxostat Impurity 94
Fosaprepitant Impurity ELS Fosaprepitant Impurity ELS
Lenvatinib Impurity 14 Lenvatinib Impurity 14 417719-45-0 C19H17ClN4O4
Lenvatinib Impurity 23 Lenvatinib Impurity 23
Olsalazine Impurity F Olsalazine Impurity F
Regorafenib Impurity 19 Regorafenib Impurity 19
Regorafenib Impurity 26 Regorafenib Impurity 26
Sitafloxacin Impurity ZZ-B-01 Sitafloxacin Impurity ZZ-B-01
Tofacitinib Impurity 3 Tofacitinib Impurity 3 1206825-36-6 C13H19N5
Tofacitinib Impurity 89 Tofacitinib Impurity 89
Nintedanib Impurity L Nintedanib Impurity L 2095596-44-2 C31H33N5O4
(2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-ethyl-3,4,10,11,13- pentahydroxy-3,5,6,8,10,12,14-heptamethyl-1-oxa-6- azacyclopentadecan-15-one (2R,3S,4R,5R,8R,10R,11R,12S,13S,14R)-2-ethyl-3,4,10,11,13- pentahydroxy-3,5,6,8,10,12,14-heptamethyl-1-oxa-6- azacyclopentadecan-15-one 117693-42-2 C22H43NO7
Rizatriptan EP Impurity A Rizatriptan EP Impurity A 887001-08-3 C28H35N7
Dapoxetine iMpurity Dapoxetine iMpurity 38262-42-9 C11H10O3S
Eltrombopag Impurity 7 Eltrombopag Impurity 7
Saxagliptin Impurity 9 Saxagliptin Impurity 9
(1r,4r)-4-((2-aminobenzyl)amino)cyclohexanol (1r,4r)-4-((2-aminobenzyl)amino)cyclohexanol 15992-77-5 C15H22N2O5
Moxifloxacin degrade Impurity Moxifloxacin degrade Impurity
Edaravone Impurity 17 Edaravone Impurity 17
Capecitabine impurity 7 Capecitabine impurity 7
Linagliptin Impurity 46 Linagliptin Impurity 46
((3R,5S,E)-3,5-dihydroxy-7-(4-isopropyl-2-(N-methylmethylsulfonamido)-6-phenylpyrimidin-5-yl)hept-6-enoate)calcium(II) ((3R,5S,E)-3,5-dihydroxy-7-(4-isopropyl-2-(N-methylmethylsulfonamido)-6-phenylpyrimidin-5-yl)hept-6-enoate)calcium(II) C44H56CaN6O12S2
Levofloxacin Impurity 24 Levofloxacin Impurity 24 C18H20FN3O4
(trans)-2-(3,4-difluorophenyl)cyclopropanecarboxamide (trans)-2-(3,4-difluorophenyl)cyclopropanecarboxamide 1932275-13-2 C10H9F2NO
(3R,5S,Z)-7-(4-(4-fluorophenyl)-6-isopropyl-2-(N-methylmethylsulfonamido)pyrimidin-5-yl)-3,5-dihydroxyhept-6-enoic acid calcium(II) (3R,5S,Z)-7-(4-(4-fluorophenyl)-6-isopropyl-2-(N-methylmethylsulfonamido)pyrimidin-5-yl)-3,5-dihydroxyhept-6-enoic acid calcium(II) 1445208-17-2 C22H28FN3O6S
Tafluprost Impurity 7 Tafluprost Impurity 7 733036-94-7 C25H35FO5
Tropisetron Impurity 1 Tropisetron Impurity 1
Fosaprepitant Impurity DBJ Fosaprepitant Impurity DBJ
Cabozantinib impurity 29 Cabozantinib impurity 29
Cabozantinib impurity 34 Cabozantinib impurity 34
Carfilzomib Impurity YGH Carfilzomib Impurity YGH
Ondansetron Impurity 3 Ondansetron Impurity 3
Irsogladine Impurity GDT Irsogladine Impurity GDT
Carvedilol Impurity NH Carvedilol Impurity NH
Pregabalin Impurity 8 Pregabalin Impurity 8 44996-52-3 C7H11NO3
Pregabalin Impurity 14 Pregabalin Impurity 14 1610546-44-5 C7H11NO3
Cefditoren Impurity 1 Cefditoren Impurity 1
Cefadroxil Impurity C Cefadroxil Impurity C 79962-91-7 C16H17N3O5S
Pralatrexate Impurity 8 Pralatrexate Impurity 8 112408-16-9 C8H15NO4
Pitavastatin Impurity 46 Pitavastatin Impurity 46
Canagliflozin Impurity 46 Canagliflozin Impurity 46
Lenvatinib Impurity 37 Lenvatinib Impurity 37
Sitafloxacin Impurity 20 Sitafloxacin Impurity 20
posaconazole impurity 66 posaconazole impurity 66
Dapoxetine impurity 33 Dapoxetine impurity 33
Budesonide Impurity 4 Budesonide Impurity 4
9-(1,3-diacetate-2-propoxymethyl)guanine 9-(1,3-diacetate-2-propoxymethyl)guanine 86357-19-9 C13H17N5O6
Cefadroxil Impurity 6 Cefadroxil Impurity 6 2378158-45-1 C32H32N6O9S2
Loratadine Impurity 9 Loratadine Impurity 9 125743-81-9 C22H23ClN2O2
Metronidazole Impurity 1 Metronidazole Impurity 1
Parecoxib Impurity 16 Parecoxib Impurity 16 2235371-89-6 C19H18N2O4S
Rosuvastatin Impurity 54 Rosuvastatin Impurity 54
Vonoprazan Fumarate Impurity 6 Vonoprazan Fumarate Impurity 6 2250243-23-1 C33H27F2N5O4S2
3-((3aS,4R,6S,6aR)-6-(2-hydroxyethoxy)-2,2-dimethyltetrahydro- 4H-cyclopenta[d][1,3]dioxol-4-yl)-5-(propylthio)-3H-[1,2,3] triazolo[4,5-d]pyrimidin-7-ol 3-((3aS,4R,6S,6aR)-6-(2-hydroxyethoxy)-2,2-dimethyltetrahydro- 4H-cyclopenta[d][1,3]dioxol-4-yl)-5-(propylthio)-3H-[1,2,3] triazolo[4,5-d]pyrimidin-7-ol 1456538-51-4 C17H25N5O5S
Esomeprazole Impurity 24 Esomeprazole Impurity 24
Prasugrel Impurity 15 Prasugrel Impurity 15
Pregabalin Impurity 18 Pregabalin Impurity 18 1621171-19-4 C9H15NO2
Pregabalin Impurity 23 Pregabalin Impurity 23
Butylphthalide impurity Butylphthalide impurity 16692-58-3 C12H14O2
ethyl 2,5-dihydroxybenzenesulfonate ethyl 2,5-dihydroxybenzenesulfonate C8H10O5S
5-ALLYLIDENE-10,11-DIHYDRO-5H-DIBENZO[A,D]CYCLOHEPTENE 5-ALLYLIDENE-10,11-DIHYDRO-5H-DIBENZO[A,D]CYCLOHEPTENE 24755-73-5 C18H16
tert-butyl (4-(benzyloxy)-8-methylnaphthalen-2-yl)carbamate tert-butyl (4-(benzyloxy)-8-methylnaphthalen-2-yl)carbamate 1662687-76-4 C23H25NO3
Nintedanib Impurity 27 Nintedanib Impurity 27
ethyl 2-(2-formyl-3-hydroxyphenyl)-4-methylthiazole-5- carboxylate ethyl 2-(2-formyl-3-hydroxyphenyl)-4-methylthiazole-5- carboxylate C14H13NO4S
LUMAT-004 LUMAT-004 313544-31-9 C16H17N3O3
Varenicline Impurity 3 Varenicline Impurity 3 1333145-89-3 C14H15N3
Amlodipine Impurity 22 (Z-Isomer) Amlodipine Impurity 22 (Z-Isomer) C26H31ClN2O8S
Amlodipine Impurity 28 Amlodipine Impurity 28 79781-21-8 C18H20ClNO5
Axitinib Impurity 16 Axitinib Impurity 16
Axitinib Impurity 22 Axitinib Impurity 22
Imidazo[5,1-f][1,2,4]triazin-4(1H)-one, 2-[2-ethoxy-5-[(4-ethyl-4-oxido-1-piperazinyl)sulfonyl]phenyl]-5-methyl-7-propyl- Imidazo[5,1-f][1,2,4]triazin-4(1H)-one, 2-[2-ethoxy-5-[(4-ethyl-4-oxido-1-piperazinyl)sulfonyl]phenyl]-5-methyl-7-propyl- 448184-48-3 C23H32N6O5S
Dolutegravir 4-Desfluoro Impurity Dolutegravir 4-Desfluoro Impurity 1863916-87-3 C20H20FN3O5
Tetrabenazine Dehydro Impurity Tetrabenazine Dehydro Impurity 100322-43-8 C19H25NO3
Cefotiam Impurity 22 Cefotiam Impurity 22 1628441-07-5 C13H14N4O5S2
Dezocine Impurity 2 Dezocine Impurity 2 100667-17-2 C16H21NO
loteprednol Impurity 7 loteprednol Impurity 7 82034-20-6 C24H33ClO7
Sitagliptin Impurity 28 Sitagliptin Impurity 28 898543-70-9 C32H25F12N9O2
Atracurium Impurity 31 Atracurium Impurity 31
Canagliflozin Impurity 49 Canagliflozin Impurity 49 2243132-56-9 C34H35FO11S
Canagliflozin Impurity 55 Canagliflozin Impurity 55
Rosuvastatin Impurity 74 Rosuvastatin Impurity 74
Tofacitinib Impurity 106 Tofacitinib Impurity 106
Hydroxychloroquine Impurity J Hydroxychloroquine Impurity J
Levetiracetam Impurity 25 Levetiracetam Impurity 25
Levosimendan Impurity 7 Levosimendan Impurity 7
Cabozantinib impurity K Cabozantinib impurity K
Crisaborole Impurity 8 Crisaborole Impurity 8
Trifluridine Impurity B Trifluridine Impurity B
Rotigotine Impurity Rotigotine Impurity
Olprinone Impurity 2 Olprinone Impurity 2 106730-62-5 C14H12N4O2
Nicorandil Impurity F Nicorandil Impurity F
Nicorandil Impurity A Nicorandil Impurity A
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